Publications

1. Lagal, DJ; Bárcena, JA; Requejo-Aguilar, R; Padilla, CA & Leto, TL. "NOX1 and PRDX6 synergistically support migration and invasiveness of hepatocellular carcinoma cells through enhanced NADPH oxidase activity". Advances in Redox Research, 2023. DOI: htt10.1016/j.arres.2023.100080.
2. Lagal, DJ; López-Grueso, MJ; Pedrajas, JR; Leto, TL; Bárcena, JA; Requejo-Aguilar, R & CA Padilla. "Loss of PRDX6 Aborts Proliferative and Migratory Signaling in Hepatocarcinoma Cell Lines". Antioxidants 12-1153, 2023. DOI: 10.3390/antiox12061153
3. Requejo-Aguilar, R (AC). "Cdk5 and aberrant cell cycle activation at the core of neurodegeneration". Neural Regeneration Research. 18(6):1186-1190, 2023.
4. López-Grueso, MJ; Padilla, CA; Bárcena, JA & Requejo-Aguilar R. "Deficiency of Parkinson's Related Protein DJ-1 Alters Cdk5 Signalling and Induces Neuronal Death by Aberrant Cell Cycle Re-entry". Cellular and Molecular Neurobiology. 2022. doi: 10.1007/s10571-022-01206-7.
5. López-Grueso, MJ; Lagal, DJ; García-Jiménez, AF; Tarradas, RM; Carmona, B; Peinado-Peinado, J; Requejo-Aguilar, R; Bárcena, JA & Padilla, CA. "Knockout of PRDX6 induces mitochondrial dysfunction and cell cycle arrest at G2/M in HepG2 hepatocarcinoma cells". Redox Biology. 37 - 101737, pp. 1 - 14, 2020. DOI:10.1016/j.redox.2020.101737.
6. González, R; Rodríguez-Hernández, MA; Negrete, M; Ranguelova, K; Rossin, A; Choya-Foces, C; Cruz-Ojeda, PDL; Miranda-Vizuete, A; Martínez-Ruiz, A; Rius-Pérez, S; Sastre, J; Bárcena, JA; Hueber, A-O; Padilla, CA & Muntané, J. "Downregulation of thioredoxin-1-dependent CD95 S-nitrosation by Sorafenib reduces liver cancer". Redox Biology. 34 - 101528, 2020. DOI: 10.1016/j.redox.2020.101510.
7. Rodríguez-Hernández, MA; Castejón-Vega, B; Cruz-Ojeda, PDL; López-Grueso, MJ; Navarro-Villarán, E; Requejo-Aguilar, R; Negrete, M; Gallego, P; Vega-Ochoa, A; Victor, VM; Cordero, MD; Del Campo, JA; Bárcena, JA; Padilla, CA & J Muntané . "Integrated molecular signaling involving mitochondrial dysfunction and alteration of cell metabolism induced by tyrosine kinase inhibitors in cancer". Redox Biology. 36 - 101510, 2020. DOI: 10.1016/j.redox.2020.101510.
8. Bonzon-kulichenko, E; Camafeita, E; López, JA; Gómez-serrano, M; Jorge-Cerrudo, I; Calvo, E; Núñez, E; Trevisan-herraz, M; Bagwan, N; Bárcena-Ruiz, JA; Peral, B & Vázquez, J. "Improved integrative analysis of the thiol redox proteome using filter-aided sample preparation". Journal of Proteomics, 214-103624, 2020. DOI: 10.1016/j.jprot.2019.103624.
9. Martina, JA, Guerrero-Gómez, D, Gómez-Orte, E, Bárcena, JA, Cabello, J, Miranda-Vizuete, A & Puertollano, R. "A conserved cysteine-based redox mechanism sustains TFEB/HLH-30 activity under persistent stress". EMBO Journal, 2020. DOI: 10.15252/embj.2020105793.
10. López-Grueso, MJ; Tarradas, RM; Carmona-Hidalgo, B; Lagal, DJ; Peinado, J; McDonagh, B; Requejo-Aguilar, R; Bárcena, JA & Padilla, CA. "Peroxiredoxin 6 Down-Regulation Induces Metabolic Remodeling and Cell Cycle Arrest in HepG2 Cells". Antioxidants. 8 - 505, pp. 1 - 17. 2019.
DOI: 10.3390/antiox8110505.
11. Padilla, CA; Bárcena, JA; López-Grueso, MJ & Requejo, R. "The regulation of TORC1 pathway by the yeast chaperones Hsp31 is mediated by SFP1 and a ff ects proteasomal activity". BBA - General Subjects. 1863, pp. 534 - 546, 2019. DOI: 10.1016/j.bbagen.2018.12.011.
12. López-Grueso, MJ; González, R; Muntané, J; Bárcena, JA & Padilla, CA. "Thioredoxin Downregulation Enhances Sorafenib Effects in Hepatocarcinoma Cells. Antioxidants". 8 - 501, pp. 1 - 18. 2019. DOI: 10.3390/antiox8100501.
13. López-Grueso, MJ; González-Ojeda, R; Requejo-Aguilar, R; McDonagh, B; Fuentes-Almagro, CA; Muntané, J; Bárcena, JA & Padilla, CA. "Thioredoxin and Glutaredoxin regulate metabolism through different multiplex thiol switches". Redox Biology. Elsevier, 2019. DOI: 10.1016/j.redox.2018.11.007.
14. Picazo, C; Mcdonagh-, B; Peinado-Peinado, J; Bárcena-Ruiz, JA; Matallana, E & Aranda, A. "Yeast cytosolic thioredoxins control glycolysis, lipid metabolism and protein biosynthesis under winemaking conditions". Applied and Environmental Microbiology, 85-7, 2019. DOI: doi:10.1128/AEM.02953-18.
15. Sottil, C; Salor-torregrosa, JM; Moreno-García, J; Peinado-Peinado, J; García-Mauricio, JC; Moreno-Vigara, JJ & García-Martínez, MT. "Using Torulaspora delbrueckii, Saccharomyces cerevisiae and Saccharomyces bayanus wine yeasts as starter cultures for fermentation and quality improvement of mead". European Food Research and Technology, 245-12, pp. 2705-2714, 2019. DOI: 10.1007/s00217-019-03384-z. IF: 2.366.
16. Fernandez-Fernandez S, Bobo-Jimenez V, Requejo-Aguilar R, Gonzalez-Fernandez S, Resch M, Carabias-Carrasco M, Ros J, Almeida A & Bolaños JP. "Hippocampal neurons require a large pool of glutathione to sustain dendrite integrity and cognitive function". Redox Biology, 19:52-61, 2018. DOI: 10.1016/j.redox.2018.08.003.
17. Pedrajas JR & Bárcena JA. "Peroxiredoxins: Types, characteristics and functions in higher plants". In Antioxidants and Antioxidant Enzymes in Higher Plants. Edited by Gupta DK, Palma JM, Corpas FJ. Springer International Publishing, 5:95-121, 2018. DOI: 10.1007/978-3-319-75088-0_6.
18. González, R; Molina-Ruiz, FJ; Bárcena, JA; Padilla, CA & Muntané, J. Regulation of Cell Survival, "Apoptosis, and Epithelial-to-Mesenchymal Transition by Nitric Oxide-Dependent Post-Translational Modifications". Antioxidant & Redox Signalling. 2018. DOI: 10.1089/ars.2017.7072.
19. Requejo-Aguilar, R; Alastrue-Agudo, A; Cases-Villar, M; López-Moncholi, E; England, R; Vicent, MJ & Moreno-Manzano, V. "Combined polymer-curcumin conjugate and ependymal progenitor/stem cell treatment enhances spinal cord injury functional recovery". Biomaterials, 113, pp. 18-30, 2017. DOI: 10.1016/j.biomaterials.2016.10.032.
20. Domínguez-Martín, MA; Gómez-Baena, G; Diez, J; López-Grueso, MJ; Beynon, RJ & García-Fernández, JM. "Quantitative Proteomics Shows Extensive Remodeling Induced by Nitrogen Limitation in ProchlorococcusmarinusSS120". American Society for Microbiology Journals, e00008-17-17, 2017. DOI: 10.1128/mSystems.00008-17.
21. Requejo-Aguilar, R & Bolaños, JP. "Mitochondrial Control of Cell Bioenergetics in Parkinson's Disease". Free Radical Biology and Medicine, 100: 123-37, 2016. DOI: 10.1016/j.freeradbiomed.2016.04.012.
22. Coppo, L; Montano, S; Padilla, CA & Holmgren, A. Determination of glutaredoxin enzyme activity and protein S-glutathionylation using fluorescent eosin-glutathione. Analytical Biochemistry, 2016. ISSN 0003-2697 DOI: 10.1016/j.ab.2016.01.012.
23. Pedrajas, JR; McDonagh, B; Hernández-Torres, F; Miranda-Vizuete, A; González, R ; Martínez-Galisteo, E; Padilla, CA & Bárcena, JA. "Glutathione is the resolving thiol for thioredoxin peroxidase activity of 1-Cys Peroxiredoxin without being consumed during the catalytic cycle". Antioxidants and Redox Signaling, 2016. ISSN 1523-0864. DOI: doi:10.1089.
24. González, R; López-Grueso, MJ; Muntané, J; Bárcena, JA & Padilla, CA. "Redox regulation of metabolic and signaling pathways by thioredoxin and glutaredoxin in NOS-3 overexpressing hepatoblastoma cells". Redox Biology. 6, pp. 122 - 134, 2015. ISSN 2213-2317. DOI: 10.1016/j.redox.2015.07.007.
25. Rodríguez-Hernández, A; Navarro-Villarán, E; González, R; Pereira, S; Soriano-DeCastro, LB; Sarrias-Giménez, A; Barrera-Pulido, L; Álamo-Martínez, JM; Serrablo-Requejo, A; Blanco-Fernández, G; Nogales-Muñoz, A; Gila-Bohórquez, A ; Pacheco, D; Torres-Nieto, MA ; Serrano-Díaz-Canedo, J; Suárez-Artacho, G; Bernal-Bellido, C ; Marín-Gómez, LM; Bárcena, JA; Gómez-Bravo, MA; Padilla, CA; Padillo, FJ & Muntané, J. "Regulation of cell death receptor S-nitrosylation and apoptotic signaling by Sorafenib in hepatoblastoma cells". Redox Biology. 6, pp. 174 - 182, 2015. DOI: 10.1016/j.redox.2015.07.010.
26. Dumitriu, D; Peinado, RA; Peinado, J & López de Lerma, N. "Grape Pomace Extract Improves the in Vitro and in Vivo Antioxidant Properties of Wines From Sun Light Dried Pedro Ximénez Grapes". Journal of Functional Foods, 17 (August): 380-87, 2015. DOI: 10.1016/j.jff.2015.06.003.
27. Padilla, CA; González, R; López-Grueso, MJ & Bárcena, JA. "Redox Regulation of Metabolic and Signaling Pathways by Thioredoxin and Glutaredoxin in Nitric Oxide Treated Hepatoblastoma Cells". Redox Biology, 5 (August):418, 2015 DOI: 10.1016/j.redox.2015.09.025.
28. Requejo-Aguilar, R; Lopez-Fabuel, I; Jimenez-Blasco, D; Fernandez, E; Almeida, A & Bolaños, JP. "DJ1 Represses Glycolysis and Cell Proliferation by Transcriptionally Up-Regulating Pink1". The Biochemical Journal, 467(2):303-10, 2015 DOI:10.1042/BJ20141025.

Other relevant publications

B. McDonagh, C.A. Padilla, J.R. Pedrajas, J.A. Barcena, Biosynthetic and iron metabolism is regulated by thiol proteome changes dependent on glutaredoxin-2 and mitochondrial peroxiredoxin-1 in Saccharomyces cerevisiae, J. Biol. Chem. 286 (2011). https://doi.org/10.1074/jbc.M110.193102.

D. Sheehan, B. McDonagh, J.A. Bárcena, Redox proteomics., Expert Rev. Proteomics. 7 (2010) 1-4. https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=20121467&retmode=ref&cmd=prlinks.

J.R. Pedrajas, C.A. Padilla, B. McDonagh, J.A. Bárcena, Glutaredoxin participates in the reduction of peroxides by the mitochondrial 1-CYS peroxiredoxin in saccharomyces cerevisiae, Antioxid. Redox Signal. 13 (2010) 249-258. https://doi.org/10.1089/ars.2009.2950.

K.F. Discola, M.A. de Oliveira, J.R. Rosa Cussiol, G. Monteiro, J.A. Bárcena, P. Porras, C.A. Padilla, B.G. Guimarães, L.E.S. Netto, Structural Aspects of the Distinct Biochemical Properties of Glutaredoxin 1 and Glutaredoxin 2 from Saccharomyces cerevisiae, J. Mol. Biol. 385 (2009).

B. McDonagh, S. Ogueta, G. Lasarte, C.A.A. Padilla, J.A.J.A. Bárcena, Shotgun redox proteomics identifies specifically modified cysteines in key metabolic enzymes under oxidative stress in Saccharomyces cerevisiae., J. Proteomics. 72 (2009) 677-689. https://doi.org/10.1016/j.jprot.2009.01.023.

P. Porras, C.A. Padilla, M. Krayl, W. Voos, J.A. Bárcena, One Single In-frame AUG Codon Is Responsible for a Diversity of Subcellular Localizations of Glutaredoxin 2 in Saccharomyces cerevisiae, J. Biol. Chem. 281 (2006) 16551-16562. https://doi.org/10.1074/jbc.M600790200.

J.R. Pedrajas, P. Porras, E. Martínez-Galisteo, C.A. Padilla, A. Miranda-Vizuete, J.A. Bárcena, Two isoforms of Saccharomyces cerevisiae glutaredoxin 2 are expressed in vivo and localize to different subcellular compartments, Biochem. J. 364 (2002) 617-623. https://doi.org/10.1042/BJ20020570.

P. Porras, J.R. Pedrajas, E. Martínez-Galisteo, C.A. Padilla, C. Johansson, A. Holmgren, J.A. Bárcena, Glutaredoxins catalyze the reduction of glutathione by dihydrolipoamide with high efficiency., Biochem. Biophys. Res. Commun. 295 (2002) 1046-1051. https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=12135599&retmode=ref&cmd=prlinks

L. García-Pardo, M.D. Granados, F. Gaytán, C.A. Padilla, E. Martínez-Galisteo, C. Morales, J.E. Sánchez-Criado, J.A. Bárcena, Immunolocalization of glutaredoxin in the human corpus luteum, Mol. Hum. Reprod. 5 (1999) 914-919. https://doi.org/10.1093/molehr/5.10.914

P. Klatt, E.P. Molina, M.G. De Lacoba, C.A. Padilla, E. Martínez-Galisteo, J.A.A. Bárcena, S. Lamas, Redox regulation of c-Jun DNA binding by reversible S-glutathiolation, FASEB J. 13 (1999) 1481-1490. https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=99393473.

C.A. Padilla, E. Martínez-Galisteo, J.A. Bárcena, G. Spyrou, A. Holmgren, Purification from Placenta, Amino Acid Sequence, Structure Comparisons and cDNA Cloning of Human Glutaredoxin, Eur. J. Biochem. 227 (1995) 27-34. https://doi.org/10.1111/j.1432-1033.1995.tb20356.x

B. Rozell, J.A. Barcena, E. Martinez-Galisteo, C.A. Padilla, A. Holmgren, Immunochemical characterization and tissue distribution of glutaredoxin (thioltransferase) from calf, Eur. J. Cell Biol. 62 (1993)

C.A. Padilla, E. Martínez-Galisteo, J. López-Barea, A. Holmgren, J.A. Bárcena, Immunolocalization of thioredoxin and glutaredoxin in mammalian hypophysis, Mol. Cell. Endocrinol. 85 (1992) 1-12. https://doi.org/10.1016/0303-7207(92)90119-Q

Publications

1. Lagal, DJ; Bárcena, JA; Requejo-Aguilar, R; Padilla, CA & Leto, TL. "NOX1 and PRDX6 synergistically support migration and invasiveness of hepatocellular carcinoma cells through enhanced NADPH oxidase activity". Advances in Redox Research, 2023. DOI: htt10.1016/j.arres.2023.100080.

2. Lagal, DJ; López-Grueso, MJ; Pedrajas, JR; Leto, TL; Bárcena, JA; Requejo-Aguilar, R & CA Padilla. "Loss of PRDX6 Aborts Proliferative and Migratory Signaling in Hepatocarcinoma Cell Lines". Antioxidants 12-1153, 2023. DOI: 10.3390/antiox12061153

3. Requejo-Aguilar, R (AC). "Cdk5 and aberrant cell cycle activation at the core of neurodegeneration". Neural Regeneration Research. 18(6):1186-1190, 2023.

4. López-Grueso, MJ; Padilla, CA; Bárcena, JA & Requejo-Aguilar R. "Deficiency of Parkinson's Related Protein DJ-1 Alters Cdk5 Signalling and Induces Neuronal Death by Aberrant Cell Cycle Re-entry". Cellular and Molecular Neurobiology. 2022. doi: 10.1007/s10571-022-01206-7.

9. Martina, JA, Guerrero-Gómez, D, Gómez-Orte, E, Bárcena, JA, Cabello, J, Miranda-Vizuete, A & Puertollano, R. "A conserved cysteine-based redox mechanism sustains TFEB/HLH-30 activity under persistent stress". EMBO Journal, 2020. DOI: 10.15252/embj.2020105793.

11. Padilla, CA; Bárcena, JA; López-Grueso, MJ & Requejo, R. "The regulation of TORC1 pathway by the yeast chaperones Hsp31 is mediated by SFP1 and a ff ects proteasomal activity". BBA - General Subjects. 1863, pp. 534 - 546, 2019. DOI: 10.1016/j.bbagen.2018.12.011.

12. López-Grueso, MJ; González, R; Muntané, J; Bárcena, JA & Padilla, CA. "Thioredoxin Downregulation Enhances Sorafenib Effects in Hepatocarcinoma Cells. Antioxidants". 8 - 501, pp. 1 - 18. 2019. DOI: 10.3390/antiox8100501.

14. Picazo, C; Mcdonagh-, B; Peinado-Peinado, J; Bárcena-Ruiz, JA; Matallana, E & Aranda, A. "Yeast cytosolic thioredoxins control glycolysis, lipid metabolism and protein biosynthesis under winemaking conditions". Applied and Environmental Microbiology, 85-7, 2019. DOI: doi:10.1128/AEM.02953-18.

17. Pedrajas JR & Bárcena JA. "Peroxiredoxins: Types, characteristics and functions in higher plants". In Antioxidants and Antioxidant Enzymes in Higher Plants. Edited by Gupta DK, Palma JM, Corpas FJ. Springer International Publishing, 5:95-121, 2018. DOI: 10.1007/978-3-319-75088-0_6.

18. González, R; Molina-Ruiz, FJ; Bárcena, JA; Padilla, CA & Muntané, J. Regulation of Cell Survival, "Apoptosis, and Epithelial-to-Mesenchymal Transition by Nitric Oxide-Dependent Post-Translational Modifications". Antioxidant & Redox Signalling. 2018. DOI: 10.1089/ars.2017.7072.

21. Requejo-Aguilar, R & Bolaños, JP. "Mitochondrial Control of Cell Bioenergetics in Parkinson's Disease". Free Radical Biology and Medicine, 100: 123-37, 2016. DOI: 10.1016/j.freeradbiomed.2016.04.012.

22. Coppo, L; Montano, S; Padilla, CA & Holmgren, A. Determination of glutaredoxin enzyme activity and protein S-glutathionylation using fluorescent eosin-glutathione. Analytical Biochemistry, 2016. ISSN 0003-2697 DOI: 10.1016/j.ab.2016.01.012.

26. Dumitriu, D; Peinado, RA; Peinado, J & López de Lerma, N. "Grape Pomace Extract Improves the in Vitro and in Vivo Antioxidant Properties of Wines From Sun Light Dried Pedro Ximénez Grapes". Journal of Functional Foods, 17 (August): 380-87, 2015. DOI: 10.1016/j.jff.2015.06.003.

27. Padilla, CA; González, R; López-Grueso, MJ & Bárcena, JA. "Redox Regulation of Metabolic and Signaling Pathways by Thioredoxin and Glutaredoxin in Nitric Oxide Treated Hepatoblastoma Cells". Redox Biology, 5 (August):418, 2015 DOI: 10.1016/j.redox.2015.09.025.

28. Requejo-Aguilar, R; Lopez-Fabuel, I; Jimenez-Blasco, D; Fernandez, E; Almeida, A & Bolaños, JP. "DJ1 Represses Glycolysis and Cell Proliferation by Transcriptionally Up-Regulating Pink1". The Biochemical Journal, 467(2):303-10, 2015 DOI:10.1042/BJ20141025.

Other relevant publications